FastQCFastQC Report
Sun 19 Mar 2023
SRR4896037_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896037_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595468
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCT1598250.0143.747731
AGATCTG1598600.0143.716252
GATCTGG588350.0142.947573
GATCTGA385250.0142.766513
GATCTGC136100.0142.730353
GATCTGT499950.0142.444643
ATCTGGA151800.0142.150194
ATCTGTA134200.0141.80034
ATCTGAG148100.0141.666444
ATCTGAT126100.0141.31644
ATCTGGT202500.0141.013344
ATCTGCG52700.0140.994314
ATCTGAA108400.0139.749084
ATCTGGG227750.0139.605714
ATCTGTG207200.0139.239384
TCTGTAG60400.0135.655625
TCTGGAT53200.0135.473685
ATCTGCA30750.0135.336584
TCTGGTA59500.0135.28745
ATCTGCT43900.0134.97954
Produced by FastQC (version 0.11.5)