FastQCFastQC Report
Sun 19 Mar 2023
SRR4896037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595468
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG61200.38358650878613676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59650.37387149099825256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC50070.31382641331571676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC26450.1657820777351849No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC25770.16152000541533895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23000.14415832846537818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT19000.11908731481922545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG18250.11438649976057182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC15960.10003334444814939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT378000.0136.112216
GGTATCA379750.0135.541858
TGGTATC380950.0135.114887
AAGCAGT381850.0134.871891
GTATCAA381800.0134.795239
AGCAGTG387250.0133.067762
AGTGGTA386950.0133.057025
CAGTGGT387600.0132.990834
GCAGTGG396800.0129.919593
TCAACGC378200.027.77535410-14
CAACGCA379650.027.71099710-14
TACATGG378850.027.63267720-24
AGAGTAC378900.027.62143315-19
ATCAACG379150.027.59562110-14
GTACATG379650.027.56686620-24
CAGAGTA381100.027.46953815-19
AGTACAT380750.027.38132920-24
CGCAGAG384350.027.21103315-19
GCAGAGT386400.027.11511615-19
GAGTACA379350.027.06103320-24
Produced by FastQC (version 0.11.5)