FastQCFastQC Report
Sun 19 Mar 2023
SRR4896036_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896036_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745910
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACAC1755450.0143.21622
GGACACT589850.0143.023093
GACACGT196850.0143.012054
AAGGACA1760650.0142.793231
GGACACG522850.0142.691383
GACACTG188800.0142.436054
GACACTA165900.0142.393814
GACACTT225100.0142.20844
GACACAT175150.0141.985344
GACACGA148150.0141.472444
GGACACA479600.0140.937063
GACACGG167350.0140.51474
ACACGTA61450.0138.609865
ACACGAT54100.0138.409975
ACACGGT60900.0137.851845
ACACGTG63850.0137.797585
ACACGTT71150.0137.523155
GACACAG151450.0137.439034
ACACTAG57400.0137.226095
CACGTAG17900.0136.357166
Produced by FastQC (version 0.11.5)