FastQCFastQC Report
Sun 19 Mar 2023
SRR4896036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745910
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG66930.383353093802086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57080.3269355235951452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC44020.2521321259400542No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC34190.19582910917515794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG33880.19405353082346743No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC28960.16587338408050814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG26930.15424620971298636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23050.13202284195634367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC19220.11008585780481238No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC19090.1093412604315228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT412250.0136.845431
GGTATCA413100.0136.659998
GTGGTAT413250.0136.54076
TGGTATC414150.0136.313527
GTATCAA415200.0135.916789
CAGTGGT421300.0134.055224
AGCAGTG421250.0133.980092
AGTGGTA422500.0133.602485
GCAGTGG436000.0129.430983
CGACCTA10200.028.09396445-49
TCAACGC412000.027.97816810-14
AGAGTAC411800.027.93580815-19
GTACATG412100.027.87353920-24
CAGAGTA412700.027.87139515-19
CAACGCA414950.027.81396710-14
TACATGG414150.027.74947520-24
ATCAACG413750.027.741510-14
AGTACAT413500.027.65728420-24
GCAGAGT420300.027.35713815-19
CGCAGAG420250.027.29186415-19
Produced by FastQC (version 0.11.5)