FastQCFastQC Report
Sun 19 Mar 2023
SRR4896035_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896035_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298839
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC1301500.0143.701262
GAGATCG410750.0143.333913
AGATCGT162450.0143.069264
AAGAGAT1308150.0142.970761
AGATCGA121650.0142.934654
GAGATCA345650.0142.916823
GAGATCT446650.0142.823243
AGATCTA128100.0142.370034
GATCGAT45600.0142.263155
AGATCAT128300.0141.979744
AGATCTG138100.0141.966694
GATCGTA48150.0141.906525
GATCGAG39350.0141.804325
AGATCAG111000.0141.64
GATCTAG41550.0141.054155
GAGATCC106350.0140.682653
GATCGTG49550.0140.51265
GATCATG38950.0140.11815
GATCGGT42650.0140.117235
GATCGTT62700.0139.636355
Produced by FastQC (version 0.11.5)