FastQCFastQC Report
Sun 19 Mar 2023
SRR4896035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298839
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG118710.9139700917511716No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC53500.41190632557229956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48190.37102366036129186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC40600.31258685641561423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA35420.272705085079829No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT26540.2043363342184828No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25080.1930955260813696No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG22600.17400155061558822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAT17490.13465872213569197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGAGAGAGGCACTTTGC13190.10155223241679685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT416700.0135.870651
GGTATCA418700.0135.210928
GTGGTAT418550.0135.207786
GTATCAA420500.0134.769129
TGGTATC420750.0134.586387
CAGTGGT423900.0133.59144
AGCAGTG426700.0132.65752
AGTGGTA428150.0132.209785
GCAGTGG434700.0130.249253
CAACGCA417800.027.7828610-14
TCAACGC417750.027.74137510-14
ATCAACG417200.027.66404310-14
AGAGTAC417450.027.65092715-19
GTACATG417800.027.59329820-24
TACATGG418600.027.58872220-24
CAGAGTA419650.027.52998715-19
AGTACAT419750.027.38620420-24
CGCAGAG421050.027.3803115-19
GCAGAGT425800.027.11883515-19
GAGTACA418000.027.00823820-24
Produced by FastQC (version 0.11.5)