FastQCFastQC Report
Sun 19 Mar 2023
SRR4896034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295328
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAC1299300.0143.556691
ACTCACC1299350.0143.551162
CTCACCG434800.0142.691823
CTCACCT417100.0142.342833
CTCACCA331200.0141.826083
TCACCGT160200.0141.707874
TCACCGA118450.0141.386254
TCACCGG147950.0140.982764
TCACCTG142600.0140.566624
TCACCTA121200.0140.495064
TCACCAG111900.0139.81774
TCACCAT122850.0138.139194
CACCGAT43300.0138.013855
CACCGTA46350.0137.475725
CACCGTG52950.0137.065165
TCACCTT153400.0137.053454
CACCGGT55250.0136.702275
CACCGTT61600.0135.935075
CACCAGT42700.0134.7265
CACCTAG39500.0134.70385
Produced by FastQC (version 0.11.5)