FastQCFastQC Report
Sun 19 Mar 2023
SRR4896034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295328
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG74360.5740630944440327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC58150.44892104548037254No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35330.27274945033227105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30370.23445799056300798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG26570.20512179154623383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC20560.15872427678549372No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC18850.14552298722794535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCCT18710.14444217989574842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG18250.14089095580424418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC16090.12421564267891994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC14560.11240396254848194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACAGTGGCTCAC13740.10607351960275697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13190.10182749079769757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT336450.0134.046846
GGTATCA338450.0133.254738
TGGTATC339600.0132.909487
AAGCAGT341200.0132.326171
AGTGGTA342300.0131.840095
GTATCAA343100.0131.453819
CAGTGGT344850.0130.953774
AGCAGTG344950.0130.889222
GCAGTGG353900.0127.599423
CAACGCA336150.027.45549610-14
TCAACGC337700.027.31668910-14
GTACATG337600.027.2341520-24
TACATGG338250.027.19884520-24
ATCAACG339350.027.08202410-14
CGCAGAG341300.026.95599415-19
AACGCAG342400.026.9543410-14
AGAGTAC342550.026.88705615-19
AGTACAT341150.026.87899820-24
CAGAGTA343750.026.8015715-19
GAGTACA338850.026.66198220-24
Produced by FastQC (version 0.11.5)