FastQCFastQC Report
Sun 19 Mar 2023
SRR4896033_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896033_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447250
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1450150.0143.712022
TTCACGC1452400.0143.48941
CACGCAT486250.0143.42253
ACGCATG152350.0142.960284
ACGCATT176950.0142.901384
ACGCAGT172200.0142.787454
ACGCATA145650.0142.66534
CACGCAG463950.0142.556753
CACGCAA391350.0142.454573
ACGCAAT139300.0142.035894
CGCATAG48550.0141.627185
CGCATGA42050.0141.26045
CGCAAGT49100.0141.213855
CGCAGTA51000.0141.176475
CGCATTG61050.0141.169535
ACGCAAG132350.0141.116734
CGCATAA43300.0140.674365
CGCATAT51900.0140.670525
CGCATTA52300.0140.558325
ACGCAGA126050.0140.515674
Produced by FastQC (version 0.11.5)