FastQCFastQC Report
Sun 19 Mar 2023
SRR4896033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447250
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG93970.649300397305234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG60840.42038348592157543No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC59940.41416479530143374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC53360.36869925721195373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45980.3177059941267922No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT32160.2222145448263949No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24340.16818103299360856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCTGGCGCGGTGGCTCA21300.147175678010019No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG19480.13460010364484365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC19480.13460010364484365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA15730.1086888927275868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT400850.0137.439821
GGTATCA403200.0136.587058
GTGGTAT403650.0136.363436
TGGTATC406200.0135.649197
GTATCAA406650.0135.486059
AGCAGTG408950.0134.628722
CAGTGGT410650.0134.245744
AGTGGTA413300.0133.196955
GCAGTGG423750.0130.007133
CAACGCA402950.027.88922910-14
TCAACGC403350.027.8294410-14
ATCAACG403050.027.73939910-14
AGAGTAC403900.027.73016515-19
CAGAGTA404900.027.66879515-19
GTACATG403850.027.63357220-24
TACATGG405700.027.54660420-24
AGTACAT404300.027.54226720-24
CGCAGAG406400.027.52769315-19
AACGCAG413650.027.17477210-14
GAGTACA404350.027.16493420-24
Produced by FastQC (version 0.11.5)