FastQCFastQC Report
Sun 19 Mar 2023
SRR4896032_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896032_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143721
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA2157150.0143.101652
GTGGTAG701200.0142.284743
GTGGTAT740050.0141.88833
TGGTGGT2180750.0141.553021
GTGGTAA577850.0141.108813
GTGGTAC158800.0140.91643
TGGTAGC117650.0139.409654
TGGTATG238950.0138.696324
TGGTATA198300.0138.625854
GGTATAC50650.0138.313455
TGGTAGT216550.0138.214254
GGTAGTC69500.0138.198095
TGGTAAG174700.0137.611434
GGTATGC71500.0136.950585
TGGTAGG220800.0136.825624
TGGTAGA170000.0136.630134
GTAGTCG23100.0136.519016
TGGTACG41200.0135.785954
GTAATCG20650.0134.934166
TGGTAAT206300.0134.855584
Produced by FastQC (version 0.11.5)