FastQCFastQC Report
Sun 19 Mar 2023
SRR4896031_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896031_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776844
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA1785950.0143.262242
TGGCTTC1787750.0143.1181
GCTTCAG593950.0142.836263
GCTTCAA468200.0141.524143
GCTTCAT618100.0140.225863
CTTCATG204750.0139.745064
CTTCAGA163500.0139.684424
CTTCAGG208650.0139.065434
CTTCAAT179100.0138.974874
CTTCAGT224250.0138.541814
GCTTCAC132050.0136.966313
CTTCAAG158800.0135.793464
TTCAGTA68200.0134.709675
CTTCATT229900.0133.978264
TTCAGAT62400.0133.153845
TCAGTAT25150.0132.834996
CTTCATA190750.0132.336564
CTTCACG43250.0131.847414
TTCATGA61400.0130.983725
TCAGATG20400.0129.882346
Produced by FastQC (version 0.11.5)