FastQCFastQC Report
Sun 19 Mar 2023
SRR4896031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776844
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG209501.1790567995839816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC142120.7998451186485702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT71700.4035244512180023No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41070.2311401563671318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37630.211779987438402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACAGTGGCTCAC36830.20727762257125557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC36400.20485760145516432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCACGGTGGCTCACG31480.17716805752221354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACGGTGGCTCAC30590.1721591766075131No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC30270.1703582306606545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCCT29800.16771309130120596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG27010.15201109382703265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC26630.14987247051513808No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCGGTGGCTCACGCCTG23350.13141277455983755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG22260.12527830242835047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC21900.12325223823813458No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA21390.12038198063532872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC19930.1121651647527864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCACAGTGGCTCACG19160.10783163856815793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA19040.10715628383808597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT18140.10209112336254618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT571600.0136.207821
GTGGTAT571850.0136.052526
TGGTATC573400.0135.659647
GGTATCA573550.0135.58658
GTATCAA575850.0134.973749
CAGTGGT583400.0133.509774
AGCAGTG583200.0133.50512
AGTGGTA588250.0132.271715
GCAGTGG596050.0130.663163
TCAACGC569500.027.87983710-14
CAACGCA570150.027.85815610-14
TACATGG571800.027.65107220-24
ATCAACG572350.027.64288710-14
GTACATG573100.027.60342420-24
AGAGTAC574950.027.5322915-19
CAGAGTA576600.027.47098515-19
CGCAGAG577250.027.4051315-19
AGTACAT577450.027.3256620-24
GCAGAGT582300.027.20949715-19
GAGTACA574250.027.04662920-24
Produced by FastQC (version 0.11.5)