FastQCFastQC Report
Sun 19 Mar 2023
SRR4896030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732040
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA736050.0143.207661
GAACAAT256050.0142.650273
GGAACAA742200.0142.021012
GAACAAG254600.0141.652793
AACAAGT93100.0138.199784
AACAATG88050.0137.621814
ACAATAG25150.0136.270375
GAACAAC60050.0134.04833
CAATATG9200.0133.043476
ACAATGT34100.0132.175955
GAACAAA187600.0132.025593
ACAATGG31600.0131.240515
ACAATGA23500.0131.131915
ACAAGTA28200.0130.978735
ACAAGAT26500.0130.68685
ACAAGTG31800.0130.64155
ACAATTG36200.0130.276255
ACAAGTT34600.0130.265885
AACAATA76850.0129.665594
CAATGAT9400.0129.446816
Produced by FastQC (version 0.11.5)