FastQCFastQC Report
Sun 19 Mar 2023
SRR4896029_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896029_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258179
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG3276000.0143.215161
GAAGAGA3290850.0142.568912
AAGAGAT1126250.0140.84813
AAGAGAG1134550.0139.462333
AAGAGAC257200.0134.229983
AAGAGAA888200.0132.618583
AGAGACG92700.0129.242524
AGAGACT86550.0128.859424
AGAGATG428150.0128.713584
GAGATAG130000.0128.492135
AGAGAGT412950.0128.272964
AGAGATA358400.0126.2207954
AGAGATT439900.0123.556994
AGAGAAT335450.0123.4375844
GAGAGTA115500.0122.867355
AGAGAGA342050.0122.550324
GAGATGT156200.0121.643865
AGAGAGG483500.0121.5882344
GAGACGT29150.0121.522985
GAGATTG166650.0121.317565
Produced by FastQC (version 0.11.5)