FastQCFastQC Report
Sun 19 Mar 2023
SRR4896029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258179
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG211900.6503632857494939No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC146740.450374273482212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG118380.3633317874800617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC113810.349305547669419No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104100.3195036245706574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA98530.3024081856767231No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT87110.26735793214553283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAC69460.21318656832543578No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA58490.17951745438172673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCAAGCGCGGTGGCTCA58440.17936399442756215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC56390.1730721363068143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACGGTGGCTCAC48530.1489482315121422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG47550.14594041641051642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGTGGTGGCTCACGCCT47480.145725572474686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC47090.14452858483220227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT43120.13234386447153457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG39610.12157097568918097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGTGGTGGCTCAC35870.11009217111767033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCCAGGCACGGTGGCTC35520.10901795143851827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA917500.0136.691968
GTGGTAT918600.0136.504756
AAGCAGT922950.0135.991071
TGGTATC923700.0135.782247
GTATCAA927250.0135.289849
CAGTGGT933450.0134.464254
AGCAGTG936850.0133.939452
AGTGGTA949500.0132.077595
GCAGTGG987700.0127.0583953
CGTGGTA3301.8189894E-1228.36325
CAACGCA917350.027.93393110-14
TCAACGC916350.027.93141210-14
ATCAACG918300.027.77330810-14
GTACATG921150.027.68027520-24
AGAGTAC922750.027.67614615-19
CAGAGTA923100.027.67189215-19
TACATGG922250.027.6347720-24
AGTACAT922350.027.5537120-24
CGCAGAG926850.027.5210915-19
GCAGAGT935150.027.333815-19
Produced by FastQC (version 0.11.5)