FastQCFastQC Report
Sun 19 Mar 2023
SRR4896028_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896028_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957591
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCACA1967800.0143.249912
TCTTCAC1969250.0143.144451
TTCACAG655600.0142.231843
TCACATG218050.0142.117864
TCACAGT243200.0142.046054
TTCACAT666250.0141.979133
TCACATA190850.0141.132834
TCACAAT194950.0140.306754
TCACAGA179750.0140.274834
TCACAGG223800.0140.107244
TCACATT247350.0139.924824
TCACAAG172400.0139.071934
TTCACAA529950.0138.484013
CACATAG63900.0137.577475
TCACACG45600.0137.210544
CACATGT74650.0136.2845
CACATGG83200.0136.211555
TTCACAC152300.0135.63233
CACAGTG86000.0135.125585
CACATGA63000.0134.628575
Produced by FastQC (version 0.11.5)