FastQCFastQC Report
Sun 19 Mar 2023
SRR4896028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957591
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG299881.5318828090239485No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC220561.1266909175614312No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60240.3077251581152549No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT40160.20515010541016995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG38190.19508671627525873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC23340.11922817381158782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT575650.0136.769061
GGTATCA578400.0136.117988
GTGGTAT577900.0136.111156
TGGTATC579350.0135.807777
GTATCAA581250.0135.478799
AGCAGTG585650.0134.511552
CAGTGGT586700.0134.346454
GCAGTGG597950.0131.744613
AGTGGTA605600.0130.02815
TCAACGC576750.027.89390810-14
CAACGCA579000.027.82281910-14
ATCAACG576050.027.82281110-14
AGAGTAC578000.027.77073115-19
GTACATG578000.027.75564220-24
CAGAGTA581150.027.65737515-19
TACATGG580100.027.64275420-24
CGCAGAG583450.027.48417715-19
AGTACAT582550.027.47211620-24
GCAGAGT588350.027.29933215-19
GAGTACA578700.027.21943520-24
Produced by FastQC (version 0.11.5)