FastQCFastQC Report
Sun 19 Mar 2023
SRR4896027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953578
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA1955400.0143.863772
CGTCTAG644700.0143.75433
CGTCTAT668250.0143.644443
CGTCTAA505150.0143.615163
TCCGTCT1959350.0143.573751
CGTCTAC140350.0143.17923
GTCTAGA168600.0143.06054
GTCTATG239000.0142.493734
GTCTAAG178350.0142.38524
GTCTAGT238200.0142.307314
GTCTAGG229000.0142.082094
GTCTATA189050.0141.905324
GTCTACG47250.0141.71434
GTCTAAT185300.0141.668664
TCTAGAT60550.0140.313785
TCTAGAG56050.0140.274755
GTCTATT236450.0139.980554
TCTAGTA67800.0139.85845
GTCTAAA142900.0138.65924
TCTATAG68950.0138.047875
Produced by FastQC (version 0.11.5)