FastQCFastQC Report
Sun 19 Mar 2023
SRR4896027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953578
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT139910.7161730936773448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG118710.6076542631008335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC93840.48034938968395424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCACGGTGGCTC91610.4689344372223684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA80740.41329294248809106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA72740.37234244038374714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68980.3530957043947055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC59420.3041598543800145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAAGTGTGGTGGCTCA51510.26367004542434447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA39750.20347280733095888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG35440.18141072432224362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCACGGTGGCTCA35400.18120597181172188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC32570.16671973169231022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC31040.15888794816485444No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACAGTGGCTCAC24510.1254621008221837No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT23530.12044566431440157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA22290.11409833648822827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC21530.1102080387883156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAAGTGTGGTGGCTCAC21320.10913308810807656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT558250.0136.796721
GTGGTAT559350.0136.545156
TGGTATC559850.0136.474627
GGTATCA559750.0136.460438
GTATCAA561100.0136.113429
AGCAGTG566750.0134.711472
CAGTGGT570450.0133.976564
AGTGGTA572250.0133.492235
GCAGTGG578050.0132.078063
CGTGGTA2303.734567E-828.1735155
TCAACGC559450.027.8409510-14
AGAGTAC559700.027.74247415-19
ATCAACG559650.027.7332210-14
CAACGCA562500.027.6976710-14
CAGAGTA561400.027.67898215-19
GTACATG561050.027.65233420-24
TACATGG562000.027.58509420-24
AGTACAT564000.027.4515320-24
CGCAGAG568050.027.3422815-19
GCAGAGT570550.027.2426615-19
Produced by FastQC (version 0.11.5)