FastQCFastQC Report
Sun 19 Mar 2023
SRR4896026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448049
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52440.36214244131241413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG45170.31193695793443454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT40760.2814821874121663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC36120.24943907284905414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG27460.18963446678945256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC22580.15593394974893804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC18330.12658411421160473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA18330.12658411421160473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC16000.1104934984934902No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC15410.10641905073654274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCCCTCGACCGCTCGCGT14760.10193025236024471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT378200.0135.903931
GTGGTAT379600.0135.348986
GGTATCA379900.0135.185248
GTATCAA380400.0135.083259
TGGTATC380850.0134.848047
AGTGGTA384200.0133.672245
AGCAGTG385750.0133.139432
CAGTGGT388450.0132.288444
GCAGTGG393550.0130.537283
CGTGGTA1952.7720125E-729.5380445
CGACCTA6900.028.17351545-49
TCAACGC378350.027.70348210-14
AGAGTAC378400.027.64273815-19
CAACGCA379950.027.62092610-14
CAGAGTA379400.027.60403615-19
TACATGG379000.027.58377620-24
GTACATG379250.027.53901520-24
ATCAACG379650.027.50620810-14
CGCAGAG381750.027.29077315-19
AGTACAT381650.027.28660420-24
Produced by FastQC (version 0.11.5)