FastQCFastQC Report
Sun 19 Mar 2023
SRR4896025_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896025_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016410
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC2018500.0143.849761
ATCAGCA2019000.0143.814152
TCAGCAT653150.0143.481483
TCAGCAG668300.0143.266973
CAGCAGT247350.0142.427724
CAGCATG224750.0142.077444
CAGCATA183200.0141.995224
TCAGCAC157700.0141.89943
TCAGCAA554000.0141.73823
CAGCATT238750.0141.677494
CAGCAAT203700.0141.419334
AGCAGTA70750.0139.928915
CAGCAAG194100.0139.733754
AGCATAG66150.0139.5375
AGCATTG89550.0138.853875
CAGCAGA177150.0138.797234
CAGCAGG241750.0138.549334
AGCAGTG83800.0138.243045
AGCATGA65400.0138.164725
AGCAATG72200.0137.916495
Produced by FastQC (version 0.11.5)