FastQCFastQC Report
Sun 19 Mar 2023
SRR4896025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016410
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG102150.5065934011436166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC86170.4273436453895785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74330.3686254283603037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT52890.26229784617215746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43930.21786243869054409No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA41520.20591050431211905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA32420.1607807935886055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA28700.14233216458954281No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC25900.12844609975153864No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC23380.11594864139733486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG21770.10796415411548246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC21710.10766659558323952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG20530.10181461111579491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT626900.0136.158581
GGTATCA627900.0135.960078
GTGGTAT627400.0135.953646
GTATCAA629500.0135.568749
TGGTATC630550.0135.3437
AGCAGTG637550.0133.979892
CAGTGGT638000.0133.811044
AGTGGTA642250.0132.810175
GCAGTGG647000.0132.023013
CGTGGTA1751.0734766E-732.913875
TCAACGC626900.027.78896110-14
AGAGTAC627750.027.6710515-19
ATCAACG627550.027.65462910-14
CAGAGTA628600.027.64966815-19
CAACGCA630550.027.64637610-14
GTACATG629100.027.58878120-24
TACATGG631700.027.45927220-24
AGTACAT631350.027.3832620-24
GCAGAGT635750.027.3273815-19
CGCAGAG636950.027.24650615-19
Produced by FastQC (version 0.11.5)