FastQCFastQC Report
Sun 19 Mar 2023
SRR4896024_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896024_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567024
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG1570300.0143.697371
AGGATGA1572900.0143.459852
GGATGAT580600.0142.68553
GGATGAG453000.0142.362923
GGATGAA434450.0141.911833
GGATGAC117550.0141.733733
GATGATA179250.0136.810044
GATGATG191100.0136.728424
GATGACG38250.0136.470584
GATGAAG143000.0135.440554
GATGAGA135400.0135.332354
GATGACT41550.0135.162454
GATGAGG156850.0134.727454
GATGAAT175700.0133.960164
GATGAGT175700.0133.796254
ATGATAG58850.0132.744285
ATGAGTA52250.0132.562685
ATGATGG69750.0132.02585
GATGATT229750.0131.966054
ATGATCG3550.0131.8315
Produced by FastQC (version 0.11.5)