FastQCFastQC Report
Sun 19 Mar 2023
SRR4896024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567024
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67180.42871072810627026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT59090.3770842054748364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG54030.3447936981182165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA53160.3392417729402996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC50410.32169258415952784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC31710.20235810045028027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG27210.17364124608174478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA26770.1708333758768213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC26040.16617486394592554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA23140.14766844668620263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG21330.13611788970685834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC17360.11078324263061702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA16560.10567802407621071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT390950.0135.212846
GGTATCA392300.0134.894388
TGGTATC392150.0134.872537
GTATCAA393050.0134.600349
AAGCAGT392700.0134.365861
AGTGGTA398950.0132.519525
CAGTGGT399300.0132.389544
AGCAGTG399350.0132.213782
GCAGTGG407400.0129.707373
TCAACGC388900.027.6109310-14
ATCAACG390450.027.44610-14
AGAGTAC391050.027.41125115-19
CAACGCA392350.027.40117310-14
CAGAGTA391550.027.37992515-19
GTACATG391000.027.3521520-24
TACATGG391000.027.33741820-24
AGTACAT393600.027.13122720-24
TACGCAC9300.027.09629650-54
GAGTACA390000.027.04198320-24
GCAGAGT397100.026.99000415-19
Produced by FastQC (version 0.11.5)