FastQCFastQC Report
Sun 19 Mar 2023
SRR4896023_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896023_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298752
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCTA1304400.0143.376272
GTTCTAG426100.0143.324113
GTTCTAT468550.0142.770663
GTTCTAA327800.0142.330693
GTGTTCT1318600.0141.832241
TCTAGTA47550.0140.214515
TTCTAGA110350.0140.085194
GTTCTAC88850.0139.543053
TTCTAGT167150.0139.52024
TTCTAGG150800.0138.795764
TCTAGTG55450.0138.676285
TCTATAG46950.0138.325885
TTCTATG166100.0138.23484
TCTAGAG37650.0137.689245
TCTATGA42900.0137.622385
TCTAGAT40500.0137.422235
TCTAATG42100.0137.330175
TCTATGG59250.0136.587365
TCTAGGT55000.0136.407275
CTAGTAG15350.0136.026066
Produced by FastQC (version 0.11.5)