FastQCFastQC Report
Sun 19 Mar 2023
SRR4896023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298752
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG107650.8288726408121027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC96580.7436369684127532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT80830.6223667028039225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44790.34486953629330314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA26100.20096215443749077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24450.18825765042132755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC23290.17932599911299463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA23010.17717008327994876No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC19480.1499901443847632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC17640.1358226974818903No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT16160.1244271423643621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC15270.1175744098950377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA14830.11418654215739417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAATCTTAGTTCAACTTTA14530.11187663233627358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG13870.1067948307298083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC13850.1066408367417336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT375000.0136.5676
GGTATCA375350.0136.554758
AAGCAGT376350.0136.236631
TGGTATC376500.0136.156787
GTATCAA376850.0135.983499
AGCAGTG381300.0134.415342
CAGTGGT383050.0133.946494
AGTGGTA386800.0132.493875
GCAGTGG392700.0130.549973
CAACGCA376000.027.7982110-14
TCAACGC375650.027.77044110-14
AGAGTAC376000.027.70841415-19
CAGAGTA376700.027.67221515-19
ATCAACG375850.027.65603810-14
GTACATG376250.027.65130420-24
TACATGG377950.027.51168620-24
AGTACAT378800.027.42714520-24
CGCAGAG381550.027.28272215-19
GAGTACA377300.027.16216720-24
GCAGAGT384900.027.1126115-19
Produced by FastQC (version 0.11.5)