FastQCFastQC Report
Sun 19 Mar 2023
SRR4896022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491811
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGTC2497950.0143.645461
TCTGTCA2498700.0143.602362
CTGTCAG806150.0143.330153
CTGTCAT860250.0143.322073
CTGTCAA666700.0142.693273
TGTCATG311300.0141.779634
TGTCAGA214950.0141.152834
TGTCAAT246150.0141.04574
TGTCAGG292200.0140.895284
TGTCAGT293700.0140.567934
TGTCATA239800.0140.547124
TGTCAAG232800.0140.350524
GTCAATG88400.0140.009055
GTCATGA80200.0139.152115
GTCAGAT74350.0138.964375
CTGTCAC179100.0138.733673
GTCAGTA90250.0138.495295
GTCATAT82250.0138.397575
TGTCATT307100.0138.302834
GTCAATA69500.0138.302155
Produced by FastQC (version 0.11.5)