FastQCFastQC Report
Sun 19 Mar 2023
SRR4896022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491811
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG171400.6878531317182563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC112900.4530841223511735No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66190.26563009794884124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT59610.2392236008268685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC57110.22919073717870259No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT46390.18616981785536704No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC43590.1749330105694212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA41270.1656225131039232No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC39690.15928174327828232No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC37410.15013177163115501No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36790.14764362144640986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA35850.14387126471469946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC33240.13339695506601423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG30840.12376540596377493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG27220.10923781940123066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC26910.10799374430885811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT664300.0136.746636
GGTATCA666300.0136.379388
TGGTATC668950.0135.81767
AAGCAGT671650.0135.43861
GTATCAA673550.0134.946189
CAGTGGT678250.0134.024374
AGCAGTG679150.0133.974142
AGTGGTA683250.0132.985555
GCAGTGG701250.0129.62873
TCAACGC662300.028.03907810-14
CAACGCA663200.028.02056910-14
ATCAACG665400.027.79591410-14
GTACATG665800.027.79379720-24
AGAGTAC666850.027.76094215-19
CAGAGTA668100.027.73486715-19
TACATGG668400.027.68999320-24
AGTACAT672050.027.42818520-24
CGCAGAG675100.027.36410115-19
GCAGAGT677550.027.33741215-19
GAGTACA665400.027.25866520-24
Produced by FastQC (version 0.11.5)