FastQCFastQC Report
Sun 19 Mar 2023
SRR4896021_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896021_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331291
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG2333750.0143.845751
TCGTAGA2333950.0143.833422
CGTAGAG713900.0143.546163
CGTAGAA632200.0143.533053
CGTAGAT832200.0143.51553
CGTAGAC161300.0142.884083
GTAGAGT242450.0140.28794
GTAGAAG210700.0139.899384
GTAGATG283350.0139.705664
GTAGAGG261050.0139.449144
GTAGATA260250.0139.435154
GTAGACT55650.0139.342324
GTAGAAT253850.0139.178254
GTAGACG53600.0139.164184
GTAGAGA211250.0139.023914
TAGATAG89100.0136.808075
GTAGATT300450.0136.379434
TAGAGTA70600.0135.637395
TAGATGG101250.0134.613335
GTAGACA42800.0133.401874
Produced by FastQC (version 0.11.5)