FastQCFastQC Report
Sun 19 Mar 2023
SRR4896021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331291
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG346931.4881454095606255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC216610.9291418360041711No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT88740.3806474609990773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67040.28756598811559775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC54490.23373315471985262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG49550.2125431788652725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG32870.14099483934009097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC30220.1296277470294356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA30100.12911301077385878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC29790.12778327544695192No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC28050.12031959974108765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG27020.11590144688071974No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC23530.10093120078102649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA678000.0136.04368
GTGGTAT679000.0135.747796
AAGCAGT680000.0135.434051
TGGTATC682100.0135.268087
GTATCAA684850.0134.685759
CAGTGGT692150.0133.247284
AGCAGTG693600.0132.871642
GCAGTGG701800.0131.360173
AGTGGTA709100.0129.985555
TCAACGC676350.027.8313710-14
CAACGCA677350.027.8072910-14
AGAGTAC678800.027.6625615-19
GTACATG678900.027.59685720-24
CAGAGTA681250.027.57364315-19
TACATGG679450.027.57027820-24
ATCAACG681000.027.54829410-14
AGTACAT682600.027.38398420-24
CGCAGAG686000.027.35542915-19
GCAGAGT692600.027.13633315-19
GAGTACA681050.027.03612120-24
Produced by FastQC (version 0.11.5)