FastQCFastQC Report
Sun 19 Mar 2023
SRR4896020_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896020_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351827
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA1353600.0143.808411
TACGCAA1353900.0143.776542
ACGCAAT467750.0143.507323
CGCAATA136200.0143.101214
ACGCAAG453150.0143.062453
CGCAAGT163750.0143.032554
CGCAATG162700.0142.982064
ACGCAAA342150.0141.474693
CGCAATT161800.0141.329944
CGCAAGA120000.0141.23994
CGCAAAT126600.0140.985674
ACGCAAC101850.0140.394593
CGCAAGG163550.0139.509544
GCAATAG48450.0139.095875
GCAAGTG57550.0138.870445
GCAAGTA49750.0138.789865
GCAATGT59450.0138.671045
GCAATTG58550.0138.343195
GCAAGAT45350.0137.966815
CGCAACT32100.0137.943824
Produced by FastQC (version 0.11.5)