FastQCFastQC Report
Sun 19 Mar 2023
SRR4896020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351827
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG95680.7077828745838041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC69550.5144889101933902No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT44140.3265210711133895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37470.27718043802942244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32510.24048935255768675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA31240.23109465930181897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC24210.1790909635626452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC22400.16570167632396748No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC21660.16022760308826497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC20820.15401379022611622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC20600.1523863630479344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG20230.14964932643008313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCACGGTGGCTCA16680.12338856969124007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC16130.11932000174578553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG15720.11628706927735576No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGAGAGAGGCACTTTGC13710.10141830278578545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA13640.10090048504727306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT418500.0134.560091
GTGGTAT419900.0133.966746
GGTATCA420550.0133.845298
TGGTATC422500.0133.21057
GTATCAA423050.0132.952219
AGCAGTG426000.0132.220112
CAGTGGT426950.0131.848684
AGTGGTA430300.0130.712145
GCAGTGG432900.0130.062743
CAACGCA418550.027.48183410-14
TCAACGC419900.027.36947310-14
GTACATG420300.027.23036820-24
AGAGTAC422150.027.192915-19
TACATGG421550.027.15645220-24
ATCAACG422500.027.08516710-14
CAGAGTA424200.027.0614915-19
CGCAGAG423250.027.0133615-19
AGTACAT423100.026.94805720-24
GCAGAGT426400.026.90497815-19
ACATGGG424250.026.74603320-24
Produced by FastQC (version 0.11.5)