FastQCFastQC Report
Sun 19 Mar 2023
SRR4896019_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896019_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131892
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA1133350.0143.808792
GGTGCGA1135000.0143.599731
TGCGAAT402000.0143.551623
TGCGAAG366500.0143.48863
GCGAATG134150.0143.301654
CGAAGTA37250.0143.032945
GCGAAGT134800.0142.931144
GCGAATA117600.0142.897344
TGCGAAA289150.0142.878863
GCGAAAT104950.0142.49014
GCGAATT143850.0142.097414
TGCGAAC80200.0141.844773
CGAATGA33350.0141.408695
CGAATTA38050.0141.350235
CGAATGT49800.0141.107825
CGAAATG36750.0141.060625
CGAATTG54550.0140.963655
CGAAGTT49350.0140.789675
CGAATAG40300.0140.783515
CGAATAT42150.0140.412225
Produced by FastQC (version 0.11.5)