FastQCFastQC Report
Sun 19 Mar 2023
SRR4896019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131892
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT122351.080933516625261No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG67770.5987320345050587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA49000.4329034925593608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC45490.4018934668678637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG43650.385637498984002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43630.3854608036809165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC40550.35824972700575675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35770.31601954956833334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21950.19392309513628508No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT20740.18323302929961516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA19780.17475165475151339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC19060.16839062384043707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA18150.16035098755004895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC17690.15628699557908352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAT13670.1207712396589074No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC13310.11759072420336923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACCCGTCTATGTA13090.11564707586942924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACACCCGTCTATGTAG12160.10743074427595564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC12050.10645892010898567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT329750.0136.187651
GTGGTAT329750.0135.984656
GGTATCA329900.0135.81378
TGGTATC331150.0135.409767
GTATCAA332050.0134.999369
AGCAGTG332850.0134.865812
CAGTGGT335900.0133.67234
AGTGGTA335800.0133.534675
GCAGTGG344200.0130.418613
CAACGCA329500.027.7813210-14
TCAACGC329500.027.7682110-14
AGAGTAC329750.027.6729215-19
CAGAGTA330400.027.6402715-19
ATCAACG329450.027.62818510-14
GTACATG330700.027.56729720-24
TACATGG331000.027.54666520-24
CGCAGAG332400.027.36999515-19
AGTACAT332050.027.3381320-24
AACGCAG335450.027.27138310-14
CGACCTA5550.027.24284245-49
Produced by FastQC (version 0.11.5)