FastQCFastQC Report
Sun 19 Mar 2023
SRR4896018_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896018_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260152
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC1266000.0143.33461
GAGAACA1267250.0143.193222
AGAACAT415000.0142.785543
GAACAGT156300.0142.065264
GAACATG148100.0142.055374
AGAACAG443100.0141.920123
GAACATA114350.0141.103644
GAACATT147650.0140.586524
GAACAAT121650.0139.916154
AGAACAA331050.0137.997283
GAACAGA121150.0136.98724
AACATAG40500.0136.355565
GAACAGG166100.0136.024084
GAACAAG115450.0135.892584
AACAGTG57500.0135.109565
AACATGT52350.0133.959885
AGAACAC102000.0133.69413
AACATTG57600.0133.3755
AACATGA42800.0132.728975
AACATGG57600.0132.3755
Produced by FastQC (version 0.11.5)