FastQCFastQC Report
Sun 19 Mar 2023
SRR4896018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260152
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG57690.4578019159593446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT47800.3793193202089907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC44530.35337006964239237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA34920.27710942806899486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34360.272665519715082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC27570.2187831309238885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA23330.1851363962442626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA20070.15926650118398414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19260.15283870517207448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC17240.13680889289546022No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC16490.1308572299214698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC15720.1247468559348396No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA14740.11697001631549211No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC14070.11165319739206063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCAGTGGCTCAC13920.11046286479726256No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC13860.10998673175934331No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC13820.10966930973406383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA299800.0135.785118
GTGGTAT300000.0135.694586
AAGCAGT300800.0135.306171
GTATCAA301400.0135.112069
TGGTATC301500.0135.091137
AGCAGTG305600.0133.2842
CAGTGGT305750.0133.21864
AGTGGTA308500.0131.955835
GCAGTGG312700.0130.303773
TCAACGC299800.027.73339810-14
AGAGTAC299650.027.70883815-19
GTACATG299200.027.68794620-24
CAGAGTA300250.027.65826215-19
CAACGCA300850.027.65096310-14
ATCAACG300750.027.55003510-14
AGTACAT301100.027.46540520-24
TACATGG302100.027.45075420-24
GCAGAGT304150.027.30834415-19
CGCAGAG303950.027.274215-19
GAGTACA299000.027.20078720-24
Produced by FastQC (version 0.11.5)