FastQCFastQC Report
Sun 19 Mar 2023
SRR4896017_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896017_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509633
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA1511350.0143.83272
GCTCGGT1512700.0143.704331
TCGGTAT501550.0143.554413
TCGGTAG484100.0143.538383
TCGGTAA413950.0143.390673
CGGTATG166000.0143.262084
CGGTATA150450.0143.138024
CGGTAGT175800.0142.893654
TCGGTAC115950.0142.633353
CGGTAGA136000.0142.411214
CGGTAAT152950.0142.398914
CGGTAGG166350.0142.095024
CGGTACG35700.0141.982644
CGGTATT178400.0141.779724
CGGTAAG139050.0141.669344
CGGTACT43050.0139.149284
CGGTAAA121400.0138.483814
GGTATAG50550.0135.738345
GGTAGAG48650.0134.083765
CGGTACA30950.0133.298364
Produced by FastQC (version 0.11.5)