FastQCFastQC Report
Sun 19 Mar 2023
SRR4896017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1509633
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG118010.7817131713469433No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT98250.6508204311908921No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC72390.4795205192255336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43100.28549985327559746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA37140.24602005917994638No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC34480.22839988262047797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCTTTTCATCTGTCC33910.22462413050059185No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA31110.20607657622746722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC30470.20183713525075297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30180.19991613855817936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA28890.1913710153394898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC26700.17686417824729586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCACGCTTTTCATCTGTC21330.1412926188020532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG20070.1329462193791471No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCACGCCTCCTCCAAGTC19620.12996536244239493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT19370.12830933081086596No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC19170.12698450550564278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG17120.11340504612710506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC16770.11108660184296448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA16560.10969553527248013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT465150.0136.850271
GTGGTAT467250.0136.185476
GGTATCA467750.0135.978338
TGGTATC468450.0135.867367
GTATCAA468800.0135.647549
CAGTGGT473650.0134.406134
AGCAGTG475300.0133.90742
AGTGGTA477900.0133.180715
GCAGTGG483600.0131.649483
TCAACGC466700.027.78847110-14
CAACGCA467950.027.7142410-14
GTACATG466450.027.70982720-24
AGAGTAC467100.027.7022815-19
ATCAACG467350.027.70052110-14
CAGAGTA467900.027.66414815-19
TACATGG467600.027.64168220-24
AGTACAT469200.027.47683320-24
CGCAGAG471200.027.46429315-19
GCAGAGT472250.027.40627915-19
ACATGGG473900.027.1982520-24
Produced by FastQC (version 0.11.5)