FastQCFastQC Report
Sun 19 Mar 2023
SRR4896016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549862
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA1551250.0143.869582
GCTAACG1551250.0143.869581
TAACGAT516500.0143.73473
TAACGAG513950.0143.593293
TAACGAC118500.0143.148913
AACGATG181550.0142.889114
TAACGAA407450.0142.780263
AACGATA153400.0142.403734
AACGAGT176700.0142.247424
ACGATAG56900.0141.848425
AACGATT176300.0141.181644
ACGATGT67500.0141.01295
ACGAGTA52250.0140.967995
AACGAAT147450.0140.923264
ACGATGA46750.0140.611315
ACGAGAT49400.0140.210085
AACGAGA142200.0139.999564
ACGATTG62200.0139.948125
CGAGTAT18350.0139.683476
ACGATTA50000.0139.535575
Produced by FastQC (version 0.11.5)