FastQCFastQC Report
Sun 19 Mar 2023
SRR4896016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549862
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG220801.4246429682126538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC126520.8163307442856202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT49360.31847996789391575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41080.2650558565859412No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA40280.25989410670111274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG29350.18937169889964395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC25550.16485338694670879No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC22120.1427223843155068No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC20230.13052775021259957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGAGAGAGGCACTTTGC20100.12968896585631495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG17260.11136475376517392No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA17170.11078405690313073No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC15780.10181551647824129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT530800.0136.965681
GGTATCA533300.0136.572168
GTGGTAT532950.0136.540256
TGGTATC534200.0136.274677
GTATCAA535950.0135.860969
AGCAGTG540450.0134.622852
CAGTGGT542850.0134.151664
GCAGTGG546100.0133.243213
AGTGGTA552250.0131.794535
CAACGCA530750.027.93220710-14
TCAACGC530850.027.92965710-14
GTACATG533100.027.72714820-24
ATCAACG532600.027.72432910-14
AGAGTAC533250.027.71682715-19
TACATGG533850.027.68819420-24
CAGAGTA534550.027.65480615-19
CGCAGAG534100.027.61070415-19
AGTACAT535650.027.48224320-24
GCAGAGT539350.027.416715-19
GAGTACA534300.027.17437220-24
Produced by FastQC (version 0.11.5)