FastQCFastQC Report
Sun 19 Mar 2023
SRR4896015_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896015_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658102
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTGT1663550.0143.528232
CACTGTG583800.0142.98873
ACCACTG1670100.0142.965331
CACTGTA470850.0142.929613
CACTGTT477000.0142.550933
ACTGTAG174600.0141.731964
CACTGTC141250.0141.553273
ACTGTAT147750.0141.124884
ACTGTCG49800.0140.963854
ACTGTGA176750.0140.863374
ACTGTTG192600.0140.44864
ACTGTAA145950.0140.448094
ACTGTGT199400.0140.316964
ACTGTGG205750.0139.38084
CTGTATG62100.0139.246385
ACTGTTA148450.0138.858874
CTGTAGT59250.0138.28865
ACTGTCA37650.0138.262954
CTGTAGG59750.0138.095385
CTGTATA43950.0136.791815
Produced by FastQC (version 0.11.5)