FastQCFastQC Report
Sun 19 Mar 2023
SRR4896015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1658102
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG118000.7116570633169733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC75980.45823477687138664No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71200.42940663481498725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG32810.19787684955449059No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC25230.1521619297244681No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA22520.13581794123642574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG18810.11344296068637515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA369800.0134.963768
AAGCAGT370850.0134.734651
GTGGTAT371450.0134.364236
TGGTATC372200.0134.132177
GTATCAA372350.0134.047559
AGCAGTG377350.0132.481282
GCAGTGG388450.0128.565863
CAGTGGT390400.0127.989734
AGTGGTA391250.0127.6196755
TCAACGC369050.027.6384510-14
CAACGCA371000.027.52035310-14
AGAGTAC370200.027.49680915-19
ATCAACG369600.027.47654210-14
CAGAGTA371750.027.44026615-19
GTACATG370600.027.42794420-24
TACATGG372000.027.30149720-24
AGTACAT371900.027.231420-24
GAGTACA371100.026.92147320-24
GCAGAGT379300.026.90165715-19
CGCAGAG378250.026.88496615-19
Produced by FastQC (version 0.11.5)