FastQCFastQC Report
Sun 19 Mar 2023
SRR4896014_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896014_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469826
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA2471850.0143.880571
CGAGTAA2472300.0143.854392
GAGTAAG857050.0142.840683
GAGTAAT841200.0142.544943
GAGTAAC179600.0141.7553
GAGTAAA625450.0140.477433
AGTAACG65100.0139.354834
AGTAATG304050.0138.885044
AGTAAGG316800.0138.363634
AGTAAGT316550.0137.81334
AGTAATA239750.0137.633384
AGTAAGA233000.0137.572544
GTAATAG86650.0136.355455
AGTAAAG247600.0135.974154
AGTAACT60250.0134.320334
AGTAATT309300.0134.223084
GTAATGA83900.0134.13115
AGTAAAT235200.0133.591844
GTAAGTG110400.0133.565225
GTAAGAG82350.0132.983615
Produced by FastQC (version 0.11.5)