FastQCFastQC Report
Sun 19 Mar 2023
SRR4896014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469826
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC142220.5758300382294137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG106810.43245961456394094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG90940.36820407591465953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC69210.2802221694969605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58340.23621097194701166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA51310.20774742836135013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT50110.20288878649751033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA43510.17616625624639143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG40020.16203570615905735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA38330.15519311886748297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCACGGTGGCTCAC36970.14968665808846454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA36750.1487959070800939No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT36500.14778369002512728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCACGGTGGCTCACG36460.14762173529633263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACCGGGCGCGGTGGCTCA35640.14430166335604208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC32410.1312238190058733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGTGCGGTGGCTCA32150.130171113268708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGGCGCAGTGGCTCA29880.12098018240961105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG29190.11818646333790317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC28790.11656691604995655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC28200.11417808380023532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC27500.11134387604632877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGCTACGGTGGCTCAT26850.10871211170341555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCGGGCGCGGTGGCTC26820.10859064565681956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACGGTGGCTCAC26440.10705207573327027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC25840.10462275480135037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT773250.0137.611771
GGTATCA778650.0136.710888
GTGGTAT778500.0136.607736
TGGTATC780050.0136.419367
GTATCAA784850.0135.718969
AGTGGTA787200.0135.107125
CAGTGGT789650.0134.78734
AGCAGTG792200.0134.311552
GCAGTGG826900.0128.698843
CAACGCA777300.028.015110-14
TCAACGC777250.028.00022910-14
ATCAACG780050.027.80372210-14
GTACATG779450.027.78704820-24
TACATGG781100.027.7375720-24
CAGAGTA785750.027.63778915-19
AGAGTAC785350.027.63536315-19
AACGCAG788100.027.61839310-14
CGCAGAG784700.027.6178815-19
AGTACAT784800.027.54258320-24
GCAGAGT792750.027.38284915-19
Produced by FastQC (version 0.11.5)