FastQCFastQC Report
Sun 19 Mar 2023
SRR4896013_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896013_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939388
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT1944500.0143.61861
CACATAG655600.0143.50583
CCACATA1947350.0143.408432
CACATAT642300.0142.217673
ACATAGG234100.0141.939354
ACATATG220450.0141.909734
ACATAGT229700.0141.398354
CACATAA513350.0141.335143
ACATAAG181950.0140.83434
ACATAGA186200.0140.287874
ACATACG51150.0139.777114
CACATAC152450.0139.607733
CATAGAT65550.0139.496575
CATAGAG63750.0139.369425
CATATGG79800.0139.03765
CATAGTG78550.0138.500325
CATAGTA66750.0138.498875
ACATATA191000.0138.270164
CATATGA59450.0137.702275
CATAGGT81200.0137.438435
Produced by FastQC (version 0.11.5)