FastQCFastQC Report
Sun 19 Mar 2023
SRR4896013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939388
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG134400.6930021223189996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC107710.5553813883555019No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57090.2943712140118429No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT49190.25363671426243745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC43390.2237303726742663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT29150.15030514780951515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG29100.15004733451996197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG27950.14411762886023838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC27060.13952855230619143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC26470.13648635548946367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG25470.13133008969839968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC23050.11885192648402486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA22860.11787223598372269No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC22820.11766598535208014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC20930.10792064300696921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT514800.0136.73896
GGTATCA516750.0136.348318
TGGTATC519000.0135.701727
GTATCAA519500.0135.543389
AAGCAGT519100.0135.504141
AGTGGTA523200.0134.59865
CAGTGGT526000.0134.036134
AGCAGTG526000.0133.933652
GCAGTGG534000.0132.008073
CGTGGTA803.8921968E-544.9992265
TCAACGC514950.027.92704610-14
CAACGCA515400.027.885910-14
GTACATG516400.027.76776320-24
AGAGTAC517850.027.69557615-19
ATCAACG517100.027.69118910-14
CAGAGTA518850.027.64774715-19
TACATGG521200.027.51480120-24
AGTACAT520350.027.47395920-24
AACGCAG523900.027.41972510-14
CGCAGAG524000.027.31556115-19
Produced by FastQC (version 0.11.5)