FastQCFastQC Report
Sun 19 Mar 2023
SRR4896012_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896012_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507517
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC1509350.0143.82351
ATAGACA1510650.0143.699742
TAGACAT491700.0143.575363
TAGACAG517650.0143.37413
AGACATG177750.0142.622794
TAGACAA393150.0142.589843
AGACAGT181600.0142.057274
AGACATA138000.0141.704354
TAGACAC114750.0141.239213
AGACAAT141400.0140.537484
AGACATT171100.0140.465234
GACATGT59050.0139.976295
GACAGTA49250.0139.76045
GACAGAT50450.0139.43315
GACATAG48550.0138.957785
GACATTG64050.0138.716635
GACATAT51000.0138.494135
GACATGA47050.0138.337945
GACATGG70350.0138.166325
GACATTA47850.0137.98125
Produced by FastQC (version 0.11.5)