FastQCFastQC Report
Sun 19 Mar 2023
SRR4896011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073354
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGA3078700.0143.749541
AGCTGAA3080350.0143.672552
GCTGAAT1073850.0143.376223
CTGAATG367500.0142.236514
GCTGAAA808400.0141.99583
CTGAATA322350.0141.676834
CTGAAGT354750.0141.523674
GCTGAAG1020300.0141.50173
CTGAATT376800.0140.3314
CTGAAGA292900.0139.943794
CTGAAAG299600.0139.457734
CTGAAAT297650.0139.210274
CTGAAGG360900.0138.493564
GCTGAAC212600.0138.44573
TGAATAG121600.0136.835335
TGAAGTA118950.0132.378115
TGAATGA104550.0131.810415
TGAATGG141950.0131.674325
TGAAGTG123350.0130.983175
TGAATTG149350.0130.886985
Produced by FastQC (version 0.11.5)