FastQCFastQC Report
Sun 19 Mar 2023
SRR4896011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073354
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG177200.5765687909690845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC126400.4112770608267059No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT112930.3674487221452524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110230.3586635317636693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79230.25779653108623346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA51000.16594248498545888No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG40170.13070411023266437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG35390.11515106948304686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC31740.10327479359683264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT31720.10320971811252462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1288950.0138.392141
GTGGTAT1303350.0136.788216
GGTATCA1304000.0136.747658
TGGTATC1306450.0136.535287
GTATCAA1308700.0136.291759
CAGTGGT1317600.0135.412734
AGCAGTG1319200.0135.237762
AGTGGTA1326400.0134.432835
GCAGTGG1336300.0133.499623
TCAACGC1302950.027.93233910-14
CAACGCA1304050.027.91650610-14
ATCAACG1304650.027.81978210-14
AGAGTAC1305000.027.80313315-19
GTACATG1306850.027.74495320-24
CAGAGTA1308300.027.74070715-19
TACATGG1308950.027.6850420-24
CGCAGAG1310900.027.65383315-19
AGTACAT1311050.027.60115420-24
GCAGAGT1317100.027.55645815-19
ACATGGG1310500.027.45780620-24
Produced by FastQC (version 0.11.5)